Annona muricata Linn Stem Bark Protects against Uterine Proliferative Disorder Induced by Estradiol Benzoate in Female Rat

Aim: The opening of mitochondrial permeability transition (mPT) pore is an important event in the execution mitochondrial-mediated apoptosis. Some bioactive compounds elicit their chemotherapeutic effects against tumor/cancer cells via induction Annona muricata, a medicinal plant, folklorically used treatment tumors and cancers. This study therefore aimed at investigating effect methanol stem bark extract muricata (MEAM) on apoptosis mPT estradiol benzoate (EB)-induced proliferative disorder using female Wistar rats.
 Methodology: Mitochondria were isolated differential centrifugation. opening, cytochrome c release ATPase activity determined spectrophotometrically. levels estrogen (E2), luteinizing hormone (LH), follicle stimulating (FSH), malondialdehyde (MDA) activities superoxide dismutase (SOD), glutathione peroxidase (GSH), ELISA technique. Histological histochemical assessments uterine sections carried out standard methods. Phytochemical constituents MEAM Gas Chromatography-Mass Spectroscopy (GC-MS).
 Results: vitro results showed significant enhancement (mATPase) concentration-dependent manner. However, oral administration did not induce rat neither any nor mATPase dosages used. Interestingly, reversed EB-induced increase E2, LH FSH. also improved antioxidant milleu by reducing MDA level increasing SOD GSH-Px groups. Administration EB induced endometrial hyperplasia model group which was mitigated group. GC-MS analysis revealed presence some phytochemicals that are pharmacological relevant cancer treatment.
 Conclusions: suggests contains protect rats.